Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan trioleate

by **stickdog99** » Wed Sep 27, 2006 11:30 pm

<a href="http://www.sciencedaily.com/releases/2006/09/060925143523.htm">www.sciencedaily.com/rele...143523.htm</a> Bird Flu Vaccine Additive May Stretch Supply Researchers have achieved an effective immune response to an avian influenza vaccine with doses as low as one-quarter of the norm when they added a chemical mixture known as MF59. The research is published in the November 1 issue of Clinical Infectious Diseases, now available online. MF 59 is an adjuvant--a substance that increases the immune system's ability to respond to a stimulus. For this research, the investigators used inactivated H9N2 influenza vaccines--not the H5N1 virus currently feared as a potential pandemic strain. However, the study does suggest that if the feared pandemic comes to be, adjuvants might be used to extend the vaccine supply. Furthermore, the authors note, H9N2 is itself a pandemic threat. <a href="http://www.scharp.org/public/redbook/protocol/apxa2.htm">www.scharp.org/public/red.../apxa2.htm</a> The adjuvant emulsion, MTP-PE/MF59, contains a muramyl tripeptide (MTP) linked covalently with dipalmitoyl phosphatidylethanolamine (PE) and MF59, a microfluidized oil-in-water emulsion. This emulsion, the adjuvant for the HIV-1 SF-2 rgp120, consists of 0.5% polysorbate 80 (Tween 80, polyoxyethylene sorbitan mono-oleate) and 0.5% sorbitan trioleate (Span 85, Arlacel 85) in a citrate buffer. Squalene (5%), a metabolizable lipid, constitutes the oil phase. For most studies, the preparation contains MF59 without MTP-PE. <a href="http://www.who.int/vaccine_safety/topics/adjuvants/squalene/Jun_2006/en/index.html">www.who.int/vaccine_safet...index.html</a> Squalene alone is not an adjuvant, but emulsions of squalene with surfactants enhance the immune response when added to antigens. MF59, a proprietary adjuvant containing squalene, is included in a seasonal subunit influenza vaccine licensed by the Italian regulatory authority in 1997 and subsequently by several other countries. The vaccine contains about 10 mg of squalene per dose. Over 22 million doses have been distributed since that time. Reported rates of adverse events and local reactogenicity are not in excess of those that would be expected with other inactivated seasonal flu vaccines, suggesting that squalene in this vaccine poses no significant risk. This vaccine has been administered primarily to individuals aged 65 years and older, for whom the vaccine was licensed. <a href="http://www.gulfwarvets.com/additive.htm">www.gulfwarvets.com/additive.htm</a> Trace amounts of the additive squalene have been found in the anthrax vaccine used to protect U.S. service members from the biological warfare agent, federal health officials have found. The finding contradicts repeated assertions by the Pentagon that squalene is not present in the vaccine. The federal Food and Drug Administration said its results were based on tests of five lots of the vaccine. The agency did not make clear whether those lots containing squalene were used to inoculate troops during the Persian Gulf War, those receiving the vaccine since a mandatory inoculation program began in 1998, or both. The FDA also did not address potential health problems with the vaccine; agency spokeswoman Lenore Gelb declined to comment. Squalene is found in the human liver, some vegetable oils and shark oil; as an additive to a vaccine, it is used to foster a faster, stronger or longer protective reaction, according to a 1999 U.S. Government Accounting Office report. It is not approved by the FDA for use in the anthrax vaccine. Squalene's safety was called into question when a 1999 Tulane University study of blood samples taken from sick gulf war veterans detected the presence of antibodies linked to the additive. Some of the samples were taken from soldiers who did not take part in the war; but all presumably received the vaccine. Previously, Congress' watchdog agency, the General Accounting Office, had reported that gulf war veterans were complaining of mysterious, undiagnosed illnesses similar to patients with auto-immune disorders. A Tennessee immunologist, Dr. Pamela B. Asa, concluded those illnesses were caused by exposure to additives in vaccines, the GAO said. James Turner, a Pentagon spokesman, said Wednesday that officials in his department were not prepared to comment on the FDA's finding. <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12042063&dopt=Abstract">www.ncbi.nlm.nih.gov/entr...t=Abstract</a> Sorbitan Laurate and Sorbitan Trioleate were cocarcinogens in one mouse study ...

by **stickdog99** » Wed Sep 27, 2006 11:33 pm

<a href="http://www.vaclib.org/basic/adjuvants.htm">www.vaclib.org/basic/adjuvants.htm</a> "When UCLA Medical School's Michael Whitehouse and Frances Beck injected squalene combined with other materials into rats and guinea pigs back in the 1970's, few oils were more effective at causing the animal versions of arthritis and multiple sclerosis", writes Matsumoto. In 1999, Dr. Johnny Lorentzen, an immunologist at Sweden's Karolinska Institute proved that on injection, "otherwise benign molecules like squalene can stimulate a self-destructive immune response", even though they occur naturally in the body. Other research institutes have also shown that the immune system makes antibodies to squalene, but only after it is injected (6) We now know that squalene, added to boost immune response in a formulation known as MF59, is the secret ingredient in certain lots of experimental anthrax vaccine that has caused devastating autoimmune diseases and death in countless Gulf War vets (Canadian, British and Australian troops were also injected with squalene laced vaccine), and continues to be used today. There is a "close match between the squalene-induced diseases in animals and those observed in humans injected with this oil: rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus", writes Matsumoto. These three illnesses have been proven to be caused by this oil, but there is an additional long list of autoimmune diseases associated with squalene injection into humans. (6) "There are now data in more than two dozen peer-reviewed scientific papers, from ten different laboratories in the U.S., Europe, Asia and Australia, documenting that squalene-based adjuvants can induce autoimmune diseases in animals..observed in mice, rats, guinea pigs and rabbits. Sweden's Karolinska Institute has demonstrated that squalene alone can induce the animal version of rheumatoid arthritis. The Polish Academy of Sciences has shown that in animals, squalene alone can produce catastrophic injury to the nervous system and the brain. The University of Florida Medical School has shown that in animals, squalene alone can induce production of antibodies specifically associated with systemic lupus erythematosus", writes Matsumoto. (6) ... As any immunologist will tell you, the way an antigen encounters the immune system makes all the difference. You can eat squalene - no problem as it is an oil the body can easily digest. But studies in animals and humans show that injecting squalene will "galvanize the immune system into attacking it, which can produce a self-destructive cross reaction against the same molecule in the places where it occurs naturally in the body - and where it is critical to the health of the nervous system." (6) Edited by: <A HREF=http://p216.ezboard.com/brigorousintuition.showUserPublicProfile?gid=stickdog99>stickdog99</A> at: 9/27/06 9:37 pm

by **stickdog99** » Thu Sep 28, 2006 12:24 am

<a href="http://home.att.net/~dstormmom/metcalf.htm">home.att.net/~dstormmom/metcalf.htm</a> Congressman Jack Metcalf has issued a report culminating a three year investigation into the conduct of the DOD (Department of Defense) with regard to the possibility that squalene, a substance in vaccine adjuvant formulations not approved by the FDA, was used in inoculations given to Gulf War era service personnel. According to the GAO (General Accounting Office), scientists have expressed safety concerns regarding the use of novel adjuvant formulations in vaccines, including squalene. The report reveals that the FDA has found trace amounts of scalene in the anthrax vaccine. The amounts recorded are enough to "boost immune response," according to immunology professor Dr. Dorothy Lewis of Baylor University. Therefore, the report concludes that immediate action should be taken to halt the current AVIP (Anthrax Vaccination Immunization Program). It further states that an aggressive investigation must be undertaken to determine the source of the squalene, and the potential health consequences to those who have been vaccinated, both during and after the Gulf War. The report also documents at length DOD "stonewalling" attempts to resolve this issue, which GAO investigators characterized as "a pattern of deception." The GAO stated the DOD denied conducting extensive squalene testing before the Gulf War, then admitted it after being confronted with the public record. The GAO revealed that DOD officials deliberating deployment of the anthrax vaccine expressed a "willingness to jump out and, use everything:" in discussing experimental vaccines containing adjuvants not approved by the FDA. GAO also found Peter Collis, DOD official who headed vaccine efforts, refused to cooperate with them. The report states that the DOD has refused to act in good faith upon the GAO recommendation to replicate the findings of a test developed by renowned virologist Dr. Robert Gary of Tulane University, although DOD admitted they could easily do so. The work of theTulane researchers has been peer-reviewed in a scientific publication of high standing. Finally, the report states that "Congress should take immediate action to review the findings of the GAO and the Armed Services Epidemiological Board, and provide independent oversight for the immediate implementation of their recommendations. "The board called on the DOD to engage in close cooperation with the Tulane researchers.

by **stickdog99** » Thu Sep 28, 2006 12:51 am

<a href="http://www.chiroweb.com/archives/18/24/06.html">www.chiroweb.com/archives/18/24/06.html</a> In a follow-up study published earlier this year, a Tennessee immunologist confirmed the findings of the Tulane study and concluded that the mysterious illnesses suffered by Gulf War veterans could have been caused by exposure to additives in vaccines.2 This conclusion was vehemently contested by Pentagon officials, who maintained that squalene was never used in the making of the anthrax vaccine, and that even it were present, it would not cause soldiers to become sick. As early as this March, the FDA began releasing preliminary information stating that low levels of squalene had indeed been detected in some anthrax vaccines. In a written statement delivered during congressional hearings into the safety of the anthrax vaccine on March 20, the FDA said that test results showed "squalene content was determined to be in a level of low parts-per-billion and was comparable to levels determined in three other lots of the anthrax vaccine."3 On September 28, the FDA released another report showing that trace amounts of squalene were found in five lots of the anthrax vaccine. The FDA did not make clear whether the lots that contained squalene were the same lots used to inoculate troops during the Gulf War, or whether they are being used in the current anthrax vaccination program. A spokesperson for the FDA also declined to comment on any potential health problems associated with the vaccine. As late as this October, however, the DoD was still insisting that squalene "is not in the anthrax vaccine" and that the substance "has not been used in vaccines ... for a considerable period of time."3 <a href="http://darwin.nap.edu/books/030907178X/html/309.html">darwin.nap.edu/books/0309...l/309.html</a> Squalene has attracted the interest of arthritis researchers because of its ability to activate the immune system nonspecifically. It was one of the constituents used in the 1970s to create the first animal models of multiple sclerosis, known as experimental allergic encephalomyelitis (EAE) (Beck et al., 1976). Squalene is one of several adjuvants (such as incomplete Freund’s adjuvant) found to induce arthritis in susceptible rat strains and has been used in the generation of animal models of arthritis (Whitehouse et al., 1974; Lorentzen, 1999). The effect is so pronounced that researchers have coined the term “squalene-induced arthritis.” After a single intraarticular injection of 50 μL squalene into Lewis (Yoshino, 1996) and Dark Agouti rats (Yoshino and Yoshino, 1994), animals experienced moderate joint inflammation by day 6, followed by more severe chronic arthritis by day 21. The inflammation was marked by joint swelling and infiltration of CD5+ and αβ+ T cells. Similarly, intradermal injection of 200 μL squalene into Dark Agouti rats produced arthritis (Lorentzen, 1999). Although the mechanisms are not fully understood, the inflammation is blocked by agents that suppress T cells (Yoshino, 1996; Sverdrup et al., 199<img src=http://www.ezboard.com/images/emoticons/glasses.gif ALT="8)"> . Animal studies do not report whether injection of squalene produces antisqualene antibodies. In summary, there is limited published information about squalene toxicity. The human relevance of what has been published is unclear because of species differences in absorption. Squalene has been found to produce arthritis and neuropathology under select conditions in animals; the relevance to humans of these toxicity findings is uncertain.

by **stickdog99** » Thu Sep 28, 2006 12:56 am

Finally, the 0.5% Polysorbate 80 is also nasty stuff. <a href="http://www.medscape.com/viewarticle/544332">www.medscape.com/viewarticle/544332</a> Docetaxel Injection (Taxotere) Linked to Risk for Severe Hypersensitivity Reactions On June 7, the FDA approved safety labeling revisions for docetaxel injection concentrate (Taxotere, made by Sanofi-Aventis US) to warn of the risk for hypersensitivity reactions associated with its use, particularly during the first and second infusions. Severe hypersensitivity reactions have been reported in patients who received the recommended 3-day dexamethasone premedication regimen (16 mg/day starting 1 day prior to docetaxel administration). The reactions were characterized by generalized rash/erythema, hypotension, and/or bronchospasm; fatal anaphylaxis occurred very rarely. Such reactions require immediate discontinuation of the infusion and initiation of appropriate therapy. Patients who experience severe hypersensitivity reactions should not be rechallenged with docetaxel. Use of the drug is also contraindicated in patients with a history of reactions to other drugs formulated with polysorbate 80.

by **Uncle Scam** » Thu Sep 28, 2006 5:51 am

<a href="http://www.youtube.com/watch?v=3CzHo-1SFdw">www.youtube.com/watch?v=3CzHo-1SFdw</a>

by **stickdog99** » Thu Sep 28, 2006 6:00 pm

<a href="http://www.newswithviews.com/Tenpenny/sherri6.htm">www.newswithviews.com/Ten...herri6.htm</a> FLU SHOTS AND THE NEW ADJUVANTS: BEWARE! Vaccination of “every man, woman and child” has been in the planning for at least the last several years. The current concept, originated by former Health and Human Services (HHS) Secretary, Tommy Thompson,[1] is being advanced by his successor, Mike Leavitt.[2] Of course, Thompson envisioned mass vaccination using the smallpox vaccine. But times have changed, and the flu shot now appears to be the instrument of choice for those pursuing the universal vaccination agenda. The fact that the flu shots are ineffective in every age group hardly seems to matter to those who continually promote their use. Multiple studies published in highly reputable publications have documented that flu shots are ineffective in all ages. For example, The Cochrane Collaboration produced a series of articles in 2005 reviewing the published literature to determine the effectiveness of the flu shot. Nothing substantiating its usefulness was found. In a review of 51 studies involving more than 260,000 children, including 17 papers translated from Russian, researchers concluded that there was “No evidence that injecting children 6-23 months of age with flu vaccines is any more effective than placebo.”[3] For healthy adults, the results were similar. A total of 25 studies were reviewed that included more than 60,000 study participants. Again, The Cochrane Group found that vaccination reduced risk of influenza by a meager 6% and reduced the number of days missed from work by less than one (0.16) day. Researchers concluded, “Universal immunization of healthy adults was not supported by the results of this review.” [4] ... An adjuvant is a substance added to produce a high antibody response using the smallest amount of virus (antigen) possible. By definition adjuvants are considered to be “pharmacologically active drugs.” They are designed to be “inert without inherent activity or toxicity” and yet they are required to “potently augment effects of the other compounds” in the vaccines.[8] It is difficult to explain how a substance can be defined as “pharmacologically active” and at the same time be described as “inert and have no activity or toxicity.” The limiting factor for approval of new adjuvants has been that most are far too toxic for use in humans. However, one adjuvant has been approved in Europe and its approval is on the way for use in the U.S. It is an oil-based adjuvant called MF-59, a compound primarily composed of squalene. On first blush, squalene seems like a good choice for an adjuvant. Manufactured naturally in the liver, squalene is a precursor for cholesterol. In addition, squalene can be purchased at health food stores in its more commonly known form, “shark liver oil.” However, ingested squalene has a completely different effect on the body than injected squalene. When molecules of squalene enter the body through an injection, even at concentrations as small as 10 to 20 parts per billion, it can lead to self-destructive immune responses, such as autoimmune arthritis and lupus.[9] Several mechanisms have been proposed to explain this reaction. Metabolically, squalene stimulates an immune response excessively and nonspecifically. More than two dozen peer-reviewed scientific papers from ten different laboratories throughout the U.S., Europe, Asia, and Australia have been published documenting the development of autoimmune disease in animals subjected to squalene-based adjuvants.[10] A convincing proposal for why this occurs includes the concept of “molecular mimicry” in which an antibody created against the squalene in MF59 can cross react with the body’s squalene on the surface of human cells. The destruction of the body’s own squalene can lead to debilitating autoimmune and central nervous system diseases. The squalene in MF59 is not the only cause for concern. One of its components, Tween80 (polysorbate 80) is considered to be “inert” but is far from it. A recent study (December 2005) discovered that Tween80 can cause anaphylaxis, a sometimes fatal reaction characterized by a sharp drop in blood pressure, hives, and breathing difficulties. Researchers concluded that the severe reaction was not a typical allergic response characterized by the combination of IgE antibodies and the release of histamines; it was caused by a serious disruption that had occurred within the immune system.[11] Vaccine manufacturer, Chiron, is already using MF59 in its European influenza vaccine for seniors called Fluad™. It remains to be seen if Chiron will gain approval for using this adjuvant-containing vaccine in the U.S. In the mean time—and for the first time—all children from age six months to five years will be targeted for the flu shot this fall. Expect even more children to be on the vaccine list as early as 2007; discussions are underway to mandatorily vaccinate the healthy five to nine year-old group as a school requirement.

Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan trioleate

Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan trioleate

Re: Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan triolea

Re: Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan triolea

Re: Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan triolea

Re: Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan triolea

Keith Olbermann receives phoney anthrax letter.

Re: Virus adjuvant MF59 = 5% Squalene, 0.5% sorbitan triolea

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